Novel diphenylazetidinones, process for their preparation, medicaments comprising these compounds and their use

ABSTRACT

Compounds of the formula I, 
     
       
         
         
             
             
         
       
     
     in which R 1 , R 2 , R 3 , R 4 , R 5 , and R 6  have the meanings given in the description, and their physiologically acceptable salts. The compounds are suitable for use, for example, as hypolipidemics.

This application claims the benefit of priority under 35 U.S.C. § 119(a)to German patent application no. 10064398.1, filed on Dec. 21, 2000, andGerman patent application no. 10152981.3, filed on Oct. 26, 2001. Thecontents of both priority documents are incorporated by referenceherein.

The invention relates to substituted diphenylazetidinones, to theirphysiologically acceptable salts and to derivatives having physiologicalfunction.

Diphenylazetidinones (such as, for example, ezetimibe) and their use fortreating hyperlipidemia and arteriosclerosis and hypercholesterolemiahave already been described [cf. Drugs of the Future 2000, 25(7):679-685and U.S. Pat. No. 5,756,470].

It was an object of the invention to provide further compounds having atherapeutically utilizable hypolipidemic action. In particular it was anobject to find novel compounds which, compared to the compoundsdescribed in the prior art, are absorbed to a very low extent. Very lowabsorption is to be understood as meaning an intestinal absorption ofless than 10%, preferably less than or equal to 5%. In particular,absorption of the novel compounds should be less than that of ezetimibe.Pharmaceutically active compounds which are absorbed to a very lowextent generally have considerably fewer side-effects.

Accordingly, an embodiment of the invention relates to compounds of theformula I,

in which

-   R1, R2, R3, R4, R5, R6 independently of one another are    (C₀-C₃₀)-alkylene-(LAG), where one or more carbon atoms of the    alkylene radical may be replaced by —O—, —(C═O), —CH═CH—, —C≡C—,    —N((C₁-C₁-C₆)-alkyl), —N((C₁-C₆)-alkylpenyl) or —NH—; or    -   H, F, Cl, Br, I, CF₃, NO₂, CN, COOH, COO(C₁-C₆) alkyl, CONH₂,        CONH(C₁-C₆)-alkyl, CON[(C₁-C₆)-alkyl]₂, (C₁-C₆)-alkyl,        (C₂-C₆)-alkenyl, (C₂-C₆)-alkynyl or O—(C₁-C₆)-alkyl, where one,        more or all hydrogens in the alkyl radicals may be replaced by        fluorine; or    -   SO₂—NH₂, SO₂NH(C₁-C₆)-alkyl, SO₂N[(C₁-C₆)-alkyl]₂,        S—(C₁-C₆)-alkyl, S—(CH₂)_(n)-phenyl, SO—(C₁-C₆)-alkyl,        SO—(CH₂)_(n)-phenyl, SO₂—(C₁-C₆)-alkyl or SO₂—(CH₂)_(n)-phenyl,        where n=0−6 and the phenyl radical may be substituted up to two        times by F, Cl, Br, OH, CF₃, NO₂, CN, OCF₃, O—(C₁-C₆)-alkyl,        (C₁-C₆)-alkyl or NH₂; or    -   NH₂, NH—(C₁-C₆)-alkyl, N((C₁-C₆)-alkyl)₂, NH(C₁-C₇-acyl, phenyl        or O—(CH₂)_(n)-phenyl, where n=0-6, where the phenyl ring may be        mono- to trisubstituted by F, Cl, Br, I, OH, CF₃, NO₂, CN, OCF₃,        O—(C₁-C₆)-alkyl, (C₁-C₆)-alkyl, NH₂, NH(C₁-C₆)-alkyl,        N((C₁-C₆)-alkyl)₂, SO₂—CH₃, COOH, COO—(C₁-C₆>alkyl or CONH₂;-   (LAG) is a sugar residue, disugar residue, trisugar residue,    tetrasugar residue;    -   a sugar acid, an amino sugar;    -   an amino acid residue, an oligopeptide residue comprising 2 to 9        amino acids; or    -   a trialkylammoniumalkyl radical; or —O—(SO₂)OH;        wherein at least one of the radicals R1 or R6 has the meaning        (C₀-C₃₀)-alkylene-(LAG), where one or more carbon atoms of the        alkylene radical may be replaced by —O—, —(C═O), —CH═CH—, —C≡C—,        —N((C₁-C₆)-alkyl), —N((C₁-C₆)-alkylpenyl) or —NH—, and where the        radicals R1 and R2 may not have the meaning —O-sugar residue or        —O-sugar acid,        and its pharmaceutically acceptable salts.

Another embodiment of the invention relates to compounds of the formulaI, in which at least one of the radicals R1 to R6 has the meaning(C₀-C₃₀) alkylene-(LAG), where one or more carbon atoms of the alkyleneradical may be replaced by —O—, —(C═O), —N((C₁-C₆)-alkyl), or —NH—.

Another embodiment of the invention relates to compounds of the formulaI, in which one of the radicals R1 or R3 has the meaning(C₀-C₃₀)-alkylene-(LAG), where one or more carbon atoms of the alkyleneradicals may be replaced by 4, —(C═O), —N(CH₃), or —NH—.

Another embodiment of the invention relates to compounds of the formulaI, in which one of the radicals R1 or R3 has the meaning—(CH₂)₀₋₁—NH—(C═O)₀₋₁—(C₀-C₂₅)-alkylene-(C═O)₀₋₁—N(R7)₀₋₁-(LAG); whereone or more carbon atoms of the alkylene radical may be replaced byoxygen atoms and where R7 is H or CH₃.

Another embodiment of the invention relates to compounds of the formulaI in which the group LAG is a monosugar residue.

A trialkylammonium alkyl radical is to be understood as meaning thefollowing group

in which n=0 to 10 and Alk₁*Alk₂, Alk₃ independently of one another eachdenote a straight-chain or branched alkyl radical having 1 to 20 carbonatoms.

Owing to their increased solubility in water, compared to the parentcompounds, pharmaceutically acceptable salts are particularly suitablefor medical applications. These salts should have a pharmaceuticallyacceptable anion or cation. Suitable pharmaceutically acceptable acidaddition salts of the compounds according to the invention are salts ofinorganic acids, such as hydrochloric acid, hydrobromic acid, phosphoricacid, metaphosphoric acid, nitric acid, sulfonic acid and sulfuric acid,and of organic acids, such as acetic acid, benzenesulfonic acid, benzoicacid, citric acid, ethanesulfonic acid, fumaric acid, gluconic acid,glycolic acid, isothionic acid, lactic acid, lactobionic acid, maleicacid, malic acid, methanesulfonic acid, succinic acid, p-toluenesulfonicacid, tartaric acid and trifluoroacetic acid, for example. For medicalpurposes, very particular preference is given to using thechloride-salt. Suitable pharmaceutically acceptable basic salts areammonium salts, alkali metal salts (such as sodium and potassium salts)and alkaline earth metal salts (such as magnesium and calcium salts).

The scope of the invention also includes salts having a pharmaceuticallyunacceptable anion, which salts may be useful intermediates forpreparing or purifying pharmaceutically acceptable salts and/or for usein nontherapeutic, for example in vitro, applications.

Here, the term “derivative having physiological function” refers to anyphysiologically acceptable derivative of a compound according to theinvention, for example an ester, capable of forming, upon administrationto a mammal, for example man, to form such a compound or an activemetabolite (directly or indirectly).

A further aspect of this invention are prodrugs of the compoundsaccording to the invention. Such prodrugs can be metabolized in vivo togive a compound according to the invention. These prodrugs may or maynot be active in their own right.

The compounds according to the invention can also be present in variouspolymorphous forms, for example as amorphous and crystallinepolymorphous forms. The scope of the invention includes all polymorphousforms of the compounds according to the invention, which form a furtheraspect of the invention.

The compounds of the invention may also exist in the form of solvates.The compounds of the formula I and their pharmaceutically acceptablesalts, esters and prodrugs and derivatives having physiological functionare ideal medicaments for treating an impaired lipid metabolism, inparticular hyperlipidemia. The compounds of the formula I are alsosuitable for modulating the serum cholesterol concentration and fortreating arteriosclerotic manifestations. The compounds of the inventionare also suitable for the treatment of insulin resistance.

As used here, “treatment” or “therapy” of a condition and “treating” acondition can mean successfully eliminating the condition, reducing theeffects associated with it, and/or reducing its severity. It alsoincludes administering the relevant compounds to a patient to avoidrecurrence of a condition. It also includes avoiding the onset of acondition by administering the relevant compounds to patients fallinginto a risk group or category for developing the particular condition.Those skilled in the art can routinely identify patients likely topresent with a given condition; thereby qualifying as candidates fortreatment.

The compound(s) of the formula (I) can also be administered incombination with other active compounds.

The amount of a compound of the formula (I) required to achieve thedesired biological effect depends on a number of factors, for example onthe specific compound chosen, on the intended use, on the mode ofadministration and on the clinical condition of the patient. In general,the daily dose is in the range from 0.1 mg to 100 mg (typically from 0.1mg to 50 mg) per day per kilogram of bodyweight, for example 0.1-10mg/kg/day. Tablets or capsules may contain, for example, from 0.01 to100 mg, typically from 0.02 to 50 mg. In the case of pharmaceuticallyacceptable salts, the abovementioned weight data relate to the weight ofthe diphenyl-azetidinone-ion derived from the salt. For the therapy ofthe above-mentioned conditions, the compounds of the formula (I) can beused themselves as the compound, but preferably they are present in theform of a pharmaceutical composition with an acceptable carrier. Thecarrier must of course be acceptable in the sense that it is compatiblewith the other constituents of the composition and is not harmful to thehealth of the patient. The carrier can be a solid or a liquid or bothand is preferably formulated with the compound as an individual dose,for example as a tablet, which can contain from 0.05% to 95% by weightof the active compound. Further pharmaceutically active substances canalso be present, including further compounds of the formula (I). Thepharmaceutical compositions according to the invention can be preparedby one of the known pharmaceutical methods, which essentially consist inmixing the constituents with pharmacologically acceptable carriersand/or auxiliaries.

Pharmaceutical compositions according to the invention are those whichare suitable for oral or peroral (e.g. sublingual) administration,although the most suitable manner of administration is dependent in eachindividual case on the nature and severity of the condition to betreated and on the type of the compound of the formula (I) used in eachcase. Coated formulations and coated delayed-release formulations arealso included in the scope of the invention. Acid-resistant and entericformulations are preferred. Suitable enteric coatings include celluloseacetate phthalate, polyvinyl acetate phthalate,hydroxypropylmethylcellulose phthalate and anionic polymers ofmethacrylic acid and methylmethacrylate.

Suitable pharmaceutical compounds for oral administration can be presentin separate units, such as, for example, capsules, cachets, lozenges ortablets, which in each case contain a specific amount of the compound ofthe formula (I); as a powder or granules; as a solution or suspension inan aqueous or nonaqueous liquid; or as an oil-in-water or water-in-oilemulsion. As already mentioned, these compositions can be preparedaccording to any suitable pharmaceutical method which includes a step inwhich the active compound and the carrier (which can consist of one ormore additional constituents) are brought into contact. In general, thecompositions are prepared by uniform and homogeneous mixing of theactive compound with a liquid and/or finely divided solid carrier, afterwhich the product, if necessary, is shaped. For example, a tablet canthus be prepared by pressing or shaping a powder or granules of thecompound, if appropriate with one or more additional constituents.Pressed tablets can be produced by tableting the compound infree-flowing form, such as, for example, a powder or granules, ifappropriate mixed with a binder, lubricant, inert diluent and/or a(number of) surface-active/dispersing agent(s) in a suitable machine.Shaped tablets can be produced by shaping the pulverulent compoundmoistened with an inert liquid diluent in a suitable machine.

Pharmaceutical compositions which are suitable for peroral (sublingual)administration include lozenges which contain a compound of the formula(I) with a flavoring, customarily sucrose and gum arabic or tragacanth,and pastiles which include the compound in an inert base such as gelatinand glycerol or sucrose and gum arabic.

Suitable other active compounds for the combination preparationsinclude:

all antidiabetics, mentioned in Rote Liste 2001, Chapter 12, thedisclosure of which is incorporated by reference herein. They can becombined with the compounds of the formula I according to the inventionin particular to achieve a synergistically enhanced action. The activecompound combination can be administered either by separateadministration of the active compounds to the patient or in the form ofcombination preparations comprising a plurality of active compounds in apharmaceutical preparation.

Antidiabetics include insulin and insulin derivatives, such as, forexample, Lantus® or HMR 1964, GLP-1 derivatives, such as, for example,those disclosed by Novo Nordisk A/S in WO 98/08871, the disclosure ofwhich is incorporated by reference herein, and oral hypoglycemic activecompounds.

The oral hypoglycemic active compounds preferably include sulfonylureas, biguadines, meglitinides, oxadiazolidinediones,thiazolidinediones, glucosidase inhibitors, glucagon antagonists, GLP-1agonists, potassium channel openers, such as, for example, thosedisclosed by Novo Nordisk AIS in WO 97/26265 and WO 99/03861, thedisclosures of which are incorporated by reference herein, insulinsensitizers, inhibitors of liver enzymes involved in stimulatinggluconeogenesis and/or glycogenolysis, modulators of glucose uptake,compounds which modulate lipid metabolism, such as antihyperlipidemicactive compounds and antilipidemic active compounds, compounds whichreduce food intake, PPAR and PXR agonists and active compounds which acton the ATP-dependent potassium channel of the beta cells.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with an HMGCoA reductase inhibitor such assimvastatin, fluvastatin, pravastatin, lovastatin, atorvastatin,cerivastatin, rosuvastatin.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with a cholesterol absorption inhibitor,such as, for example, ezetimibe, tiqueside, pamaqueside.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with a PPAR gamma agonist, such as, forexample, rosiglitazone, pioglitazone, JTT-501, GI 262570.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with a PPAR alpha agonist, such as, forexample, GW 9578, GW 7647.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with a mixed PPAR alpha/gamma agonist, suchas, for example, GW 1536, AVE 8042, AVE 8134, AVE 0847.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with a fibrate, such as, for example,fenofibrate, clofibrate, bezafibrate.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with an MTP inhibitor, such as, for example,Bay 13-9952, BMS-201038, R-103757.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with a bile acid absorption inhibitor, suchas, for example, HMR 1453.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with a CETP inhibitor, such as, for example,Bay 194789.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with a polymeric bile acid adsorber, suchas, for example, cholestyramine, colesolvam.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with an LDL receptor inducer, such as, forexample, HMR1171, HMR1586.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with an ACAT inhibitor, such as, forexample, avasimibe.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with an antioxidant, such as, for example,OPC-14117.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with a lipoprotein lipase inhibitor, suchas, for example, NO-1886.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with an ATP citrate lyase inhibitor, suchas, for example, SB-204990.

In one embodiment of the invention, the compound the formula I areadministered in combination with a squalene synthetase inhibitor, suchas, for example, BMS-188494.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with a lipoprotein(a) antagonist, such as,for example, CI-1027 or nicotinic acid.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with a lipase inhibitor, such as, forexample, Orlistat.

In one embodiment of the invention, the compounds of the formula I areadministered in combination with insulin.

In one-embodiment, the compounds of the formula I are administered incombination with a sulfonyl urea, such as, for example, tolbutamide,glibenclamide, glipizide or gliclazide.

In one embodiment, the compounds of the formula I are administered incombination with a biguanide, such as, for example, metformin.

In another embodiment, the compounds of the formula I are administeredin combination with a meglitinide, such as, for example, repaglinide.

In one embodiment, the compounds of the formula I are administered incombination with a thiazolidinedione, such as, for example,troglitazone, ciglitazone, pioglitazone, rosiglitazone, or the compoundsdisclosed by Dr. Reddy's Research Foundation in WO 97/41097, thedisclosure of which is incorporated by reference herein, in particular5-[[4-[(3,4-dihydro-3-methyl-4-oxo-2-quinazolinylmethoxy]phenyl]methyl]-2,4-thiazolidinedione.

In one embodiment, the compounds of the formula I are administered incombination with an α-glucosidase inhibitor, such as, for example,miglitol or acarbose.

In one embodiment, the compounds of the formula I are administered incombination with an active compound which acts on the ATP-dependentpotassium channel of beta cells, such as, for example, tolbutamide,glibenclamide, glipizide, gliazide or repaglinide.

In one embodiment, the compounds of the formula I are administered incombination with more than one of the abovementioned compounds, forexample in combination with a sulfonyl urea and metformin, a sulfonylurea and acarbose, repaglinide and metformin, insulin and a sulfonylurea, insulin and metformin, insulin and troglitazon, insulin andlovastatin, etc.

In a further embodiment, the compounds of the formula I are administeredin combination with CART agonists, NPY agonists, MC4 agonists, orexinagonists, β3 agonists, TNF agonists, CRF agonists, CRF BP antagonists,urocortin agonists, β3-agonists, MSH (melanocyte-stimulating hormone)agonists, CCK agonists, serotonin reuptake inhibitors, mixed serotoninand noradrenergic compounds, 5HT agonists, bombesin agonists, galaninantagonists, growth hormone, growth hormone-releasing compounds, TRHagonists, decoupling protein 2- or 3 modulators, leptin agonists, DAagonists (bromocriptin, doprexin), lipase/amylase inhibitors, PPARmodulators, RXR modulators or TR-β agonists.

In one embodiment of the invention, the further active compound isleptin.

In one embodiment, the further active compound is dexamphetamine oramphetamine.

In one embodiment, the further active compound is fenfluramine ordexfenfluramine.

In another embodiment, the further active compound is sibutramine.

In one embodiment, the further active compound is Orlistat.

In one embodiment, the further active compound is mazindol orphentermine.

In one embodiment, the compounds of the formula I are administered incombination with fiber, preferably insoluble fiber, such as, forexample, Caromax®. The combination with Caromax® can be given in onepreparation or by separate administration of compounds of the formula Iand Caromax®. Here, Caromax® can also be administered in the form offood, such as, for example, in bakery goods or muesli bars. Compared tothe individual active compounds, the combination of compounds of theformula I with Caromax® is, in addition to an enhanced action, inparticular with respect to the lowering of LDL cholesterol, alsocharacterized by its improved tolerability.

It goes without saying that each suitable combination of the compoundsaccording to the invention with one or more of the compounds mentionedabove and optionally one or more further pharmacologically activesubstances is included in the scope of the present invention.

The invention furthermore provides both stereoisomer mixtures of theformula I and the pure stereoisomers of the formula I, and diastereomermixtures of the formula I and the pure diastereomers. The mixtures areseparated by chromatographic means.

Preference is given to both racemic and enantiomerically pure compoundsof the formula I of the following structure:

Sugar residues are to be understood as meaning compounds which arederived from aldoses and ketoses which have 3 to 7 carbon atoms and maybelong to the D or the L series; also included are amino sugars, sugaralcohols or sugar acids. Glucose, mannose, fructose, galactose, ribose,erythrose, glycerolaldehyde, sedoheptulose, glucosamine, galactosamine,glucuronic acid, galacturonic acid, gluconic acid, galactonic acid,mannonic acid, glucamine, 3-amino-1,2-propanediol, glucaric acid andgalactaric acid may be mentioned by way of example.

Disugars are saccharides composed of two sugar units. Di-, tri- ortetrasaccherides are formed by acetal-like binding of two or moresugars. Here, the bonds may be in the α- or β-form. Lactose, maltose andcellobiose may be mentioned by way of, example.

If the sugar is substituted, the substitution is preferably at thehydrogen atom of an OH group of the sugar.

Suitable protective groups for the hydroxyl groups of the sugars aresubstantially: benzyl, acetyl, benzoyl, pivaloyl, trityl,tert-butyldimethylsilyl, benzylidene, cyclohexylidene or isopropylideneprotective groups.

The term “amino acids” or “amino acid residues” refers, for example, tothe stereoisomeric forms, i.e. the D or L forms, of the followingcompounds:

alanine glycine proline cysteine histidine glutamine aspartic acidisoleucine arginine glutamic acid lysine serine phenylalanine leucinethreonine tryptophan methionine valine tyrosine asparagine 2-aminoadipicacid 2-aminoisobutyric acid 3-aminoadipic acid 3-aminoisobutyric acidbeta-alanine 2-aminopimelic acid 2-aminobutyric acid 2,4-diaminobutyricacid 4-aminobutyric acid desmosine piperidine carboxylic acid2,2-diaminopimelic acid 6-aminocaproic acid 2,3-diaminopropionic acid2-aminoheptanoic acid N-ethylglycine 2-(2-thienyl)glycine3-(2-thienyl)alanine penicillamine sarcosine N-ethylasparagineN-methylisoleucine hydroxylysine 6-N-methyllysine allo-hydroxylysineN-methylvaline 3-hydroxyproline norvaline 4-hydroxyproline norleucineisodesmosine ornithine allo-isoleucine

N-methylglycine

For abbreviating the amino acids, the conventional notation was used(cf. Schröder, Lübke, The Peptides, Volume I, New York 1965, pagesXXII-XXIII; Houben-Weyl, Methoden der Organischen Chemie [Methods oforganic chemistry], Volume XV/1 and 2, Stuttgart 1974). The amino acidpGlu denotes pyroglutamyl, NaI denotes 3-(2-naphthyl)alanine, azagly-NH₂denotes a compound of the formula NH₂NH—CONH₂ and D-Asp denotes the Dform of aspartic acid. According to their chemical nature, peptides areacid amides, and on hydrolysis they decompose into amino acids.

An oligopeptide is to be understood as meaning a peptide constructed of2 to 9 of the amino acids mentioned above.

Suitable protective groups (see, for example, T. W. Greene, “ProtectiveGroups in Organic Synthesis”) for amino acids are primarily:

Arg(Tos), Arg(Mts), Arg(Mtr), Arg(PMV), Asp(OBzl), Asp(OBut),Cys(4-MeBzl), Cys(Acm), Cys(SBut), GIu(Obzl), Glu(Obut), His(Tos),His(Fmoc), His(Dnp), His(Trt), Lys(Cl-Z), Lys(Boc), Met(O), Ser(Bzl),Ser(But), Thr(Bzl), Thr(But), Trp(Mts), Trp(CHO), Tyr(Br-Z), Tyr(Bzl) orTyr(But).

Amino protective groups that are preferably used are thebenzyloxycarbonyl (Z) radical, which can be removed by catalytichydrogenation, the 2-(3,5-dimethyloxyphenyl)propyl(2)oxycarbonyl(Ddz) ortrityl (Trt) radical, which can be removed by weak acids, and the9-fluorenylmethyloxycarbonyl (Fmoc) radical, which can be removed usingsecondary amines.

The invention furthermore relates to a process for preparingdiphenylazetidinone derivatives of formula I.

Independently of one another, x and y can be from 0 to 10. In compoundII, —(CH₂)x-NH₂ may alternatively also be attached to one of the othertwo phenyl rings.

The process for preparing the compounds of the formula I comprisesreacting an amine of the formula II with an alkylating or acylatingagent which, preferably in the omega position, carries a furtherfunctionality—if appropriate in protected form. This functionality is(after deprotection) used for attaching (LAG), for example with theformation of ether, amine or amide bonds.

The examples below serve to illustrate the invention in more detail,without limiting the invention to the products and embodiments describedin the examples.

EXAMPLE IN-4-[3-(3-Hydroxy-3-phenylpropyl)-2-(4-methoxyphenyl-4-oxoazetidin-1-yl]-benzyl-5-(2,3,4,5,6-pentahydroxyhexylamino)pentanamide(3)

a)N-4-[3-(3-Hydroxy-3-phenylpropyl)-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]-benzyl-5-bromopentanamide(2)

416 mg of1-(4-aminomethylphenyl)-3-(3-hydroxy-3-phenylpropyl)-4-(4-methoxy-phenyl)azetidin-2-one(1) are dissolved in 10 ml of dried dichloromethane, and 0.2 ml oftriethylamine is added. With ice-cooling, 200 mg of 5-bromovalerylchloride, dissolved in 2 ml of dichloromethane, are added, and themixture, stirred at room temperature for 5 hours. 5 ml of water areadded, the mixture is acidified using 0.5 N HCl (pH ˜3), the phases areseparated, the aqueous phase is washed with a little dichloromethane,the combined organic solutions are dried with sodium sulfate and theresidue is, after removal of the solvent, purified by silica gel columnfiltration. This gives 2 as an oil of molecular weight 579.54(C₃₁H₃₅BrN₂O₄) MS (FAB): 581/579 (M+H⁺).

b)N-4-[3-(3-Hydroxy-3-phenylpropyl)-2-(4-methoxyphenyl-4-oxoazetidin-1-yl]benzyl-5-(2,3,4,5,6-pentahydroxyhexylamino)pentanamide(3)

300 mg of 2 are dissolved in 10 ml of dimethylformamide, and 191 mg of6-aminohexane-1,2,3,4,5-pentaol are added. The mixture is stirred at 80°C. until the reaction (monitored by thin-layer chromatography) hassubstantially ended (after about 2 hours). The solvent is then removedunder reduced pressure and the residue is chromatographed on silica gel(mobile phase: CH₂Cl₂/methanol/conc. ammonia=30:10:2). This gives 3 ofmolecular weight 679.82 (C₃₇H₄₉N₃O₉); MS (FAB): 680 (M+H⁺).

EXAMPLE IIN-4-1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-1-oxoazetidin-2-yl}benzyl-2,3,4,5,6-pentahydroxyhexanamide(4)

a)4-[5-(4-Fluorophenylyl)-(4-fluorophenylamino)-5-hydroxy-2-(2-oxo-4-phenyl-oxazolidin-3-carbonyl)pentyl]benzonitrile(5)

Under argon, 2.5 g of3-[5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-oxazolidin-2-one aredissolved in 30 ml of dichloromethane, 3.9 g of4-[4-fluoro-phenylimino)methyl]benzonitrile are added and the mixture iscooled to −10° C. 6.4 ml of diisopropylethylamine are added to thismixture and then, over a period of 3 min. 4.05 ml of trimethylsilylchloride such that the temperature does not exceed −5° C. At thistemperature, the mixture is stirred for another hour, and it is thencooled to −25° C. 0.8 ml of titanium tetrachloride is then added slowly.The dark mixture is stirred at −25 to −30° C. overnight and thendecomposed using 35 ml of 7% strength tartaric acid solution, andstirring is continued at room temperature for 1 hour. 15 ml of a 20%strength sodium bicarbonate solution are then added, and the mixture isstirred for another hour. Following phase separation, the org. phase iswashed with 30 ml of water, dried over magnesium sulfate andconcentrated to about 10 ml. After addition of 2 ml ofbistrimethylsilylacetamide, the mixture is heated at reflux for 30 minand then concentrated under reduced pressure. The residue iscrystallized with ethyl acetate/heptane. The product is filtered offwith suction and dried under reduced pressure. This gives 5 of molecularweight 653.81 (C₃₇H₃₇F₂N₃O₄Si); MS (ESI+): 654.3 (M+H⁺), 582.2(M+H⁺-Si(CH₃)₃).

b){1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzonitrile(6)

2 g of 5 are dissolved in 20 ml of methyl tert-butyl ether and, with 100mg of tetrabutylammonium fluoride trihydrate and 1.3 ml ofbistrimethylsilylacetamide, heated at 40° C. for about 1 h. The reactionis monitored by thin-layer chromatography. After the reaction has ended,initially 0.2 ml of glacial acetic acid is added, and the mixture isstirred for 30 min and concentrated. The residue is treated with 20 mlof a mixture of isopropanol/2N sulfuric acid=10:1 and stirred for 1hour. Following the addition of a spatula tip of solid sodiumbicarbonate, the mixture is once more concentrated under reducedpressure, the residue is taken up in ethyl acetate, the org. phase iswashed with water and dried and the residue is, after removal of thesolvent, purified by column chromatography (SiO₂,CH₂Cl₂/methanol=100:1). This gives 6 of molecular weight 418.45(C₂₅H₂₀F₂N₂O₂); MS (DCl+): 419 (M+H⁺).

c)4-(4-Aminomethylphenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]azetidin-2-one(7)

200 mg of 6 are dissolved in 20 ml of ethanol and, with 0.5 ml of conc.ammonia, hydrogenated over Raney nickel at a hydrogen pressure of 75 barand at 25° C. for 30 hours. The catalyst is filtered off with suction,the filtrate is concentrated under reduced pressure and the residue ispurified by column filtration (SiO₂, CH₂Cl₂/methanol/conc.NH₃=100:10:1). This gives 7 of molecular weight 422.5 (C₂₅H₂₂F₂N₂O₂); MS(DCl+): 423 (M+H⁺), 405 (M+H⁺-H₂O).

d)N-4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzyl-2,3,4,5,6-pentahydroxyhexanamide(4)

50 mg of 7 and 25 mg of3,4,5-trihydroxy-6-hydroxymethyltetrahydropyran-2-one are dissolved in 5ml of methanol and, together with 10 mg of Na₂CO₃, stirred overnight.The mixture is filtered off with suction, the filtrate is concentratedunder reduced pressure and the residue is purified by column filtration(SiO₂, CH₂Cl₂/methanol=10:1). This gives 4 having a melting point above180° C. and the molecular weight 600.6 (C₃₁H₃₄F₂N₂O₈); MS (ESI+): 601(M+H⁺), 583 (M+H⁺-H₂O).

EXAMPLE IIIN-4-[3-[3-(4-Fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-yl]benzyl-12-(2,3,4,5,6-pentahydroxyhexanoylamino)dodecanamide(8)

a) 12-(2,3,4,5,6-Pentahydroxyhexanoylamino)dodecanoic acid (9)

3.5 g of 12-aminododecanoic acid are dissolved in 500 ml of methanoland, with 2.7 g of finely powdered sodium carbonate and 4.8 g of3,4,5-trihydroxy-6-hydroxymethyltetrahydropyran-2-one, stirred at roomtemperature for 30 hours. The mixture is filtered off, the filtrate isconcentrated and the residue is dissolved in 70 ml of water. Withice-cooling, 1N hydrochloric acid is added gradually until the pH is 1-2(about 50-55 ml). The free acid precipitates out and is filtered offwith suction, washed with a little cold water, and dried under highvacuum at 35° C. This gives 9 of molecular weight 393.48 (C₁₈H₃₅NO₈); MS(ESI+): 394 (M+H⁺); (ESI−):392 (M−H)⁻.

b)N-4-[3-[3-(4-Fluorophenyl)-3-hydtoxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzyl-12-(2,3,4,5,6-pentahydroxyhexanoylamino)dodecanamide(8)

is prepared similarly to Example II, starting from1-(4-aminomethylphenyl)-(3-hydroxy-3-phenylpropyl)-4-(4-methoxyphenyl)azetidin-2-one.This gives N-4-[3-(3hydroxy-3-phenylpropyl)-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzyl-12-(2,3,4,5,6-pentahydroxyhexanoylamino)dodecanamideof melting point 100° C. and molecular weight 792 (C₄₄H₆₁N₃O₁₀); MS(ESI+): 792 (M+H⁺).

3-[5-(tert-Butyldimethylsilanyloxy)-5-(4-fluorophenyl)pentanoyl]-phenyloxazolidin-2-one(10)

30 g of3-[5-(4-Fluorophenyl)-5-hydroxypentanoyl]-4-phenyloxazolidin-2-one aredissolved in 50 ml of DMF. 14.3 g of imidazole and 19 g oftert-butyldimethylsilyl chloride in 25 ml of DMF are added, and themixture is then stirred at room temperature until the reaction has goneto completion (2-4 h). The reaction solution is concentrated, water isadded and the mixture is extracted with ethyl acetate. The organic phaseis dried over magnesium sulfate and concentrated, giving 10:

C₂₆H₃₄FNO₄Si (471.65) MS (ESI) 494 (M+Na)

3-[(4-Fluorophenylimino)methyl]benzonitrile (11)

88 ml of para-fluoroaniline are added dropwise to 12 g ofmeta-cyanobenzaldehyde in 60 ml of isopropanol. After 1 h at 60° C., theproduct precipitates out. The mixture is allowed to warm to roomtemperature and filtered off, and the residue is washed withisopropanol. Drying gives 11 of m.p. 101° C.

C₁₄H₉FN₂ (224.24).

3-[5-(tert-Butyldimethylsilanyloxy)-5-(4-fluorophenyl)-1-(4-fluorophenylamino)-2-(2-oxo-4-phenyloxazolidine-3-carbonyl)pentyl]benzonitrile(12)

At 10° C., 24 ml of diisopropylethylamine are added to 14 g of3-[5-(tert-butyldimethyl-silanyloxy)-5-(4-fluorophenyl)pentanoyl]-4-phenyloxazolidin-2-one(1 and 12.5 g of 3-[(4-fluorophenylimino)methyl]benzonitrile 11 in 200ml of methylene chloride, and 7.1 ml of trimethylsilyl chloride areadded dropwise. After 1 h, 3.4 ml of titanium tetrachloride are addeddropwise at −10° C. The mixture is stirred at −10° C. for 3 h and thenallowed to stand at −30° C. for another 12 h. 8 ml of acetic acid and140 ml of a 7% strength aqueous tartaric acid solution are then added,and stirring is continued at room temperature for another 2 h. 50 ml of20% strength aqueous sodium hydrogen sulfite solution are added, and themixture is then stirred for another hour and extracted with methylenechloride. The organic phase is dried over magnesium sulfate,concentrated and purified by silica gel chromatography (ethylacetate/heptane 1/3->1/1). This gives 12

C₄₀H₄₃F₂N₃O₄Si (695.89) MS (ESI) 696 (M+H)

3-[3-[3-(tert-Butyldimethylsilanyloxy)-3-(4-fluorophenyl)propyl]-1-(4-fluorophenyl)-4-oxoazetidin-2-yl]benzonitrile(13)

Under argon, a mixture of 13 g of3-[5-(tert-butyldimethylsilanyloxy)-5-(4-fluorophenyl)-1-(4-fluorophenylamino)-2-(2-oxo-4-phenyloxazolidine-3-carbonyl)pentyl]benzonitrile12, 50 ml of bistrimethylsilylacetamide, 0.5 g of tetrabutylammoniumfluoride and 100 ml of tert-butyl methyl ether is stirred at roomtemperature for 10 h. After the reaction has ended, 5 ml of acetic addare added slowly with ice-cooling, and the mixture is concentrated. Theresidue is separated by silica gel chromatography (ethylacetate/heptane=⅛). This gives 13:

C₃H₃₄F₂N₂O₂Si (532.71) MS (ESI) 555 (M+Na)

3-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}-benzonitrile(14)

10 ml of 1N hydrochloric acid are added to 7.8 g of3-[3-[3-(tert-butyldimethyl-silanyloxy)-3-(4-fluorophenyl)propyl]-1-(4-fluorophenyl)-4-oxoazetidin-2-yl]benzonitrile(13) in 200 ml of methanol, and the mixture is stirred for 12 h. Aqueoussodium bicarbonate solution is added to the reaction mixture, which isthen extracted with methylene chloride. The organic phase is dried overmagnesium sulfate, concentrated and purified by silica gelchromatography (ethyl acetate/heptane=1/3->1/1). This gives 14:C₂₅H₂₀F₂N₂O₂ (418.45) MS (ESI) 401 (M+H-H₂O)

EXAMPLE IV4-(3-Aminomethylphenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyopyl]-azetidin-2-one(15)

In an autoclave, at a hydrogen pressure of 75 bar, 2.5 g of3-{1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzonitrile5 are reacted in 100 ml of ethanol and 15 ml of concentrated ammoniawith 1.0 g of Raney-Nickel for 20 h. The reaction solution is filtered,concentrated and separated by silica gel chromatography (methylenechloride/methanol=10/1). This gives 15: C₂₅H₂₄F₂N₂O₂ (422.48) MS (ESI)405 (M+H—H₂O)

EXAMPLE VN-3-{2-(4-Fluorophenyl)-4-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}-benzyl-2,3,4,5,6-pentahydroxyhexanamide(16)

25 mg of sodium carbonate are added to a solution of 100 mg of4-(3-aminomethyl-phenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]azetidin-2-one6 and 46 mg of 3,4,5-trihydroxy-6-hydroxymethyltetrahydropyran-2-one in5 ml of methanol, and the mixture is stirred at room temperature untilthe reaction has gone to completion. The reaction solution is filteredand concentrated. The residue is purified by HPLC (KnauerEurospher-100-10-C18, water (0.1% trifluoroacetic acid)/acetonitrile(0.1% trifluoroacetic acid)=80/20->10/90). This gives 16:

C₃₁H₃₄F₂N₂O₈ (600.62) MS (ESI) 601 (M+H)

EXAMPLE VI[3-(3-{2-(4-Fluorophenyl)-4-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}-benzylcarbamoyl)propyl]trimethylammonium2,2,2-trifluoroacetate (17)

A solution of 100 mg of 4-(3-aminomethylphenyl)-1-(4-fluorophenyl)-3-[(4fluorophenyl)-3-hydroxypropyl]azetidin-2-one (15), 64 mg of3-carboxypropyl-trimethylammonium chloride, 93 μl ofdiisopropylcarbodiimide, 65 mg of hydroxybenzotriazole and 60 μl ofdiisopropylethylamine in 2 ml of methylene chloride is stirred at roomtemperature for 12 h. Water is added, and the mixture is extracted withmethylene chloride. The organic phase is dried over magnesium sulfate,concentrated and separated by HPLC (Knauer Eurospher-100-10-C18, water(0.1% trifluoroacetic acid)/acetonitrile (0.1% trifluoroaceticacid)=80/20->10/90). This gives 17: C₃₂H₃₅F₂N₃O₃ (550.67) MS (ESI) 551:(M+H)

EXAMPLE VII[3-(3-{2-(4-Fluorophenyl)-4-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxazetidin-2-yl}benzylcarbamoyl)-2-hydroxypropyl]trimethylammonium2,2,2-trifluoroacetate (18)

18 is prepared similarly to 17 starting from 100 mg of4-(3-aminomethylphenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]azetidin-2-one15, 64 mg of (3-carboxy-2-hydroxypropyl)trimethylammonium chloride, 93μl diisopropylcarbodiimide, and 65 mg of hydroxybenzotriazole in 2 ml ofmethylene chloride. Without any extraction step, the reaction solutionis concentrated and then purified by HPLC (Merck-Hibar-Lichrospher100-RP-18, water (0.1% trifluoroacetic acid)/acetonitrile (0.1%trifluoroacetic acid)=80/20->10/90). This gives 18:

C₃₂H₃₈F₂N₃O₄ (566.67) MS (ESI) 567 (M+H)

EXAMPLE VIII

N-[5-(3-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}phenylcarbamoyl)pentyl]-2,3,4,5,6-pentahydroxyhexanamide(19)

19 is prepared similarly to 18 starting from 100 mg of4-(3-aminomethylphenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]azetidin-2-one15,108 mg of 6-(2,3,4,5,6-pentahydroxyhexanoylamino)hexanoic acid, 93 μlof diisopropylcarbodiimide and 65 mg of hydroxybenzotriazole in 2 ml ofmethylene chloride. This gives 10:

C₃₇H₄₅F₂N₃O₉ (713.78) MS (ESI) 714 (M+H)

{2-[2-(2,3,4,5,6-Pentahydroxyhexanoylamino)ethoxy]ethoxy}acetic acid(20)

172 mg of sodium carbonate are added to a solution of 450 mg of[2-(2-aminoethoxy)ethoxy]acetic acid and 318 mg of3,4,5-trihydroxy-hydroxymethyltetrahydropyran-2-one in 10 ml ofmethanol, and the mixture is stirred at room temperature until thereaction has gone to completion. The reaction solution is filtered andconcentrated. The residue is taken up in water and acetonitrile (1/1),resulting in the formation of 2 phases. The aqueous phase isconcentrated and contains 20:

C₁₂H₂₃NO₁₀ (341.32) MS (ESI) 342 (M+H)

EXAMPLE IX

N-(2-{2-[(3-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzylcarbamoyl)methoxy]ethoxy}ethyl)-2,3,4,5,6-pentahydroxyhexanamide(21)

21 is prepared similarly to 18 starting from 100 mg of4-(3-aminomethylphenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]azetidin-2-one(15), 122 mg of{2-[2-(2,3,4,5,6-pentahydroxyhexanoylamino)ethoxy]ethoxy}acetic acid(20), 93 μl of diisopropylcarbodiimide and 65 mg of hydroxybenzotriazolein 2 ml of dimethylformamide. This gives 21: C₃₇H₄₅F₂N₃O₁₁ (745.78) MS(ESI) 746 (M+H)

EXAMPLE XN-(2-{2-[(4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzylcarbamoyl)methoxy]ethoxy}ethyl)-2,3,4,5,6-pentahydroxyhexanamide(22)

22 is prepared similarly to 18 starting from 100 mg of4-(4-aminomethylphenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]azetidin-2-one,122 mg of{2-[2-(2,3,4,5,6-pentahydroxyhexanoylamino)ethoxy]ethoxy}acetic acid 20,93 μl of diisopropylcarbodiimide and 65 mg of hydroxybenzotriazole in 2ml of dimethylformamide and 1 ml of acetonitrile. This gives 22:

C₃₇H₄₅F₂N₃O₁₁ (745.78) MS (ESI) 746 (M+H)

2,3,4-Triacetoxy-1-{2-[2-(2-aminoethoxy)ethoxy]acetyl}-5-hydroxypentylacetate (23)

In a hydrogenation apparatus, a suspension of 1.12 g of2,3,4-triacetoxy-1-{2-[2-(2-azido-ethoxy)ethoxy]acetyl}5-hydroxypentylacetate and 1.0 g of Raney-Nickel in 100 ml of ethanol is shaken underan atmosphere of hydrogen for 4 h. The reaction solution is filtered andconcentrated. The residue contains 23:

C₂₀H₃₃NO₁₂ (479.49) MS (ESI) 480 μM+H)

{2-[2-({2-[2-(3,4,5,6-Tetraacetoxy-7-hydroxy-2-oxoheptyloxy)ethoxy]ethylcarbamoyl}-methoxy)ethoxy]ethoxy}aceticacid (24)

A solution of 500 mg of2,3,4-triacetoxy-1-{2-[2-(2-aminoethoxy)ethoxy]acetyl}5 hydroxypentylacetate 23, 1.15 g of [2-(2-carboxymethoxyethoxy)ethoxy]acetic acid, 400μl of diisopropylcarbodiimide and 288 mg of hydroxybenzotriazole in 20ml of methylene chloride is stirred at room temperature for 12 h. Thereaction solution is concentrated and separated by HPLC (KnauerEurospher-100-10-C18, water (0.1% trifluoroacetic acid)/acetonitrile(0.1% trifluoroacetic acid)=80/20->10/90). This gives 24: C₂₈H₄₅NO₁₈(683.67) MS (ESI) 684 (M+H)

[2-({2-[2-(3,4,5,6-Tetraacetoxy-7-hydroxy-2-oxoheptyloxy)ethoxy]ethylcarbamoyl}-methoxy)ethoxy]aceticacid (25)

25 is prepared similarly to 24 starting from 500 mg of2,3,4-triacetoxy-1-{2-[2-(2-aminoethoxy)ethoxy]acetyl}-5-hydroxypentylacetate 23, 927 mg of (2-carboxymethoxyethoxy)acetic acid, 400 μl ofdiisopropylcarbodiimide and 288 mg of hydroxybenzotriazole in 20 ml ofmethylene chloride. This gives 25:

C₂₆H₄₁NO₁₇ (639.61) MS (ESI) 640 (M+H)

{2-[2-({2-[2-(3,4,5,6,7-Pentahydroxy-2-oxoheptyloxy)ethoxy]ethylcarbamoyl}-methoxy)ethoxy]ethoxy}aceticacid (26)

At room temperature, 100 μl of a 5.4 M sodium methoxide solution inmethanol are added to a solution of 200 mg of{2-[2-({2-[2-(3,4,5,6-tetraacetoxy-7-hydroxy-2-oxo-heptyloxy)ethoxy]ethylcarbamoyl}methoxy)ethoxy]ethoxy}aceticacid (24) in 5 ml of methanol, and the mixture is stirred for 2 h. 1 gof Amberlite IR 120 is added to the reaction solution and the mixture isstirred for 10 min, filtered and concentrated, giving 26:

C₂₀H₃₇NO₁₄ (515.52) MS (ESI) 516 (M+H)

[2-({2-[2-(3,4,5,67-Pentahydroxy-2-oxoheptyloxy)ethoxy]ethylcarbamoyl}methoxy)-ethoxy]aceticacid (27)

27 is prepared similarly to 26 starting from 200 mg of 25. This gives27:

C₂₆H₄₁N₁O₁₇ (471.46) MS (ESI) 472 (M+H)

EXAMPLE XIN-(4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}-benzyl)-2-{2-[2-({2-[2-(3,4,5,6,7-pentahydroxy-2-oxoheptyloxy)ethoxy]ethyl-carbamoyl}methoxy)ethoxy]ethoxy}acetamide(28)

28 is prepared similarly to 18 starting from 62 mg of4-(4-aminomethylphenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]azetidin-2-one,76 mg of{2-[2-({2-[2-(3,4,5,6,7-pentahydroxy-2-oxoheptyloxy)ethoxy]ethylcarbamoyl}methoxy)ethoxy]ethoxy}aceticacid 17, 57 μl of diisopropylcarbodiimide and 40 mg ofhydroxybenzotriazole in 2 ml of dimethylformamide. This gives 19:

C₄₅H₅₉F₂N₃O₁₄ (919.98) MS (ESI) 920 (M+H)

EXAMPLE XIN-(3-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}-benzyl)-2-{2-[2-({2-[2-(3,4,5,6,7-pentahydroxy-2-oxo-heptyloxy)ethoxy]ethylcarbamoyl}methoxy)ethoxy]ethoxy}acetamide (29)

29 is prepared similarly to 18 starting from 62 mg of4-(3-aminomethylphenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-hydroxypropyl]azetidin-2-one15, 76 mg of{2-[2-({2-[2-(3,4,5,6,7-pentahydroxy-2-oxoheptyloxy)ethoxy]ethylcarbamoyl}methoxy)ethoxy]ethoxy}aceticacid 26, 57 μl of diisopropylcarbodiimide and 40 mg ofhydroxybenzotriazole in 2 ml of dimethylformamide. This gives 19:

C₄₅H₅₉F₂N₃O₁₄ (919.98) MS (ESI) 920 (M+H)

EXAMPLE XIIIN-(4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}-benzyl)-2-[2-({2-[2-(3,4,5,6,7-pentahydroxy-2-oxoheptyloxy)ethoxy]-ethylcarbamoyl}-methoxy)ethoxy]acetamide(30)

30 is prepared similarly to 18 starting from 68 mg of4-(4-aminomethylphenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]azetidin-2-one76 mg of[2-({2-[2-(3,4,5,6,7-pentahydroxy-2-oxoheptyloxy)ethoxy]ethylcarbamoyl}methoxy)ethoxy]aceticacid (27, 62 μl of diisopropylcarbodiimide and 44 mg ofhydroxybenzotriazole in 2 ml of dimethylformamide. This gives 30:C₄₃H₅₅F₂N₃O₁₄ (875.93) MS (ESI) 876 (M+H)

EXAMPLE XIVN-(3-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}-benzyl)-2-[2-({2-[2-(3,4,5,6,7-pentahydroxy-2-oxoheptyloxy)ethoxy]ethylcarbamoyl}-methoxy)ethoxy]acetamide(31)

31 is prepared similarly to 18 starting from 68 mg of4-(3-aminomethylphenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]azetidin-2-one(15), 76 mg of[2-({2-[2-(3,4,5,6,7-pentahydroxy-2-oxoheptyloxy)ethoxy]ethylcarbamoyl}methoxy)-ethoxy]aceticacid (27), 62 μl of diisopropylcarbodiimide and 44 mg ofhydroxybenzotriazole in 2 ml of dimethylformamide. This gives 31:C₄₃H₅₅F₂N₃O₁₄ (875.93) MS (ESI) 876 (M+H)

EXAMPLE XV[3-(4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzylcarbamoyl)propyl]trimethylammoniumtrifluoroacetate (32)

91 mg of (3-carboxypropyl)trimethylammonium chloride are dissolved in 5ml of dimethylformamide, and the solution is cooled to 0° C. 0.055 ml ofN-methylmorpholine, 210 mg of4-(4-aminomethylphenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]azetidin-2-one,77 mg of N-hydroxybenzotriazole and 96 mg of1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride are addedsuccessively, and the reaction solution is warmed to room temperatureand stirred for 12 h. The reaction mixture is concentrated under reducedpressure and the residue is taken up in sat. sodium bicarbonatesolution, stirred and concentrated under reduced pressure. Repeatedly,this residue is stirred in acetone and the suspension is filtered. Thecombined filtrates are concentrated and purified chromatographically(RP18; acetonitrile/water ½, with 0.1% trifluoroacetic acid). This gives[3-(4-{1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzylcarbamoyl)propyl]trimethylammonium;trifluoroacetate of molecular weight 550.67 (C₃₂H₃₈F₂N₃O₃; cation); MS(ESI): 551.24 (M+H⁺).

EXAMPLE XVI

Dodecyl-[3-(4-{1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzylcarbamoyl)propyl]dimethylammoniumtrifluoroacetate (33)

The compound of Example XVI is obtained like that of Example XV, withthe difference that, instead of (3-carboxypropyl)trimethylammoniumchloride, (3-carboxypropyl)dodecyldimethylammonium chloride is used.This givesdodecyl-[3-(4-{1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzylcarbamoyl)propyl]dimethylammoniumtrifluoroacetate of molecular weight 703.96 (C₄₃H₆₉F₂N₃O₃; cation); MS(ESI): 704.70 (M+H⁺).

EXAMPLE XVIIDodecyl-[10-(4-{1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzylcarbamoyl)decyl]dimethylammoniumtrifluoroacetate (34)

The compound of Example XVII is obtained like that of Example XV, withthe difference that, instead of (3-carboxypropyl)trimethylammoniumchloride, (10-carboxydecyl)dodecyldimethylammonium chloride is used.This givesdodecyl-[10-(4-{1-(4-fluorophenyl)-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzylcarbamoyl)decyl]dimethylammoniumtrifluoroacetate of molecular weight 803.16 (C₅₀H₇₄F₂N₃O₃; cation); MS(ESI): 803.77 (M⁺).

EXAMPLE XVIII

Benzyl-(4-{4-[3-[3-(4-fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzylcarbamoyl}butyl)-(2,3,4,5,6-pentahydroxyhexyl)ammoniumtrifluoroacetate (35) a) Methyl5-[benzyl-(2,3,4,5,6-pentahydroxyhexyl)amino]pentanoate (36)

At room temperature, 1.37 g of 6-benzylaminohexane-1,2,3,4,5-pentanolare suspended in 30 ml of dry dimethylformamide, 1.45 g of potassiumcarbonate, 0.83 g of potassium iodide and 0.86 ml of methyl5-bromovalerate are added and the mixture is stirred at room temperatureovernight. The next day, the reaction mixture is filtered and thefiltrate is concentrated under reduced pressure and, for purification,subjected to chromatography (silica gel; ethyl acetate/methanol/water5/1/0.1). This gives methyl5-[benzyl-(2,3,4,5,6-pentahydroxyhexyl)amino]-pentanoate of molecularweight 385.46 (C₁₉H₃₁NO₇); MS (ESI): 386.33 (M+H⁺).

b) 5-[Benzyl-(2,3,4,5,6-pentahydroxyhexyl)amino]pentanoic acid (37)

At room temperature, 0.46 g of methyl5-[benzyl(2,3,4,5,6-pentahydroxyhexyl)-amino]pentanoate is dissolved ina mixture of 5 ml of ethanol and 5 ml of water, 0.4 g of potassiumhydroxide is added and the mixture is stirred at 80° C. for 2 h. Thecooled reaction mixture is then concentrated under reduced pressure andthe residue is taken up in water, neutralized with hydrochloric acid andagain concentrated. The crude product is suspended in ethanol, thesuspension is filtered and the filtrate is concentrated under reducedpressure. This gives5-[benzyl-(2,3,4,5,6-pentahydroxyhexyl)amino]pentanoic acid of molecularweight 371.43 (C₁₈H₂₉NO₇); MS (ESI): 372.2 (M+H⁺).

c)3-[5-(tert-Butyldimethylsilanyloxy)-5-(4-fluorophenyl)pentanoyl]-4-phenyloxazolidin-2-one(38)

27 g of3-[(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyloxazolidin-2-one, 13.6 gof tert-butyldimethylsilyl chloride and 10.2 g of imidazole aredissolved in 36 ml of dimethylformamide, and the mixture is stirred at60° C. for 90 min. After the reaction has ended, the mixture isdissolved in ethyl acetate and extracted twice with water. The organicphase is dried over magnesium sulfate, filtered and concentrated underreduced pressure. This gives3-[5-(tert-butyldimethylsilanyloxy)-5-(4-fluorophenyl)-pentanoyl]-4-phenyloxazolidin-2-oneof molecular weight 471.65 (C₂₆H₃₄FNO₄Si); MS (ESI): 340.28(MH⁺-HOSi(CH₃)₂C(CH₃)₃).

d)4-[5-(tert-Butyldimethylsilanyloxy)-5-(4-fluorophenyl)-1-(4-methoxyphenyl)-2-(2oxo-4-phenyloxazolidine-3-carbonyl)pentylamino]benzonitrile (39)

16.2 g of3-[5-(tert-butyldimethylsilanyloxy)-5-(4-fluorophenyl)pentanoyl]-4-phenyl-oxazolidin-2-oneare dissolved in 350 ml of dichloromethane. 19.8 ml of Hünig base and10.14 g of 4-[(4-methoxyphenylamino)methyl]benzonitrile are added, andthe solution is cooled to −10° C. 85.2 ml of trimethylsilyl triflate areadded to the cooled solution, which is stirred at −10° C. for 30 min.The solution is then cooled to −30° C., and 44 ml of titaniumtetrachloride solution are added. The reaction mixture is stirred atfrom −30 to 40° C. for 2 h. The reaction solution is then allowed towarm to room temperature and washed successively with 200 ml of 2Nsulfuric-acid, 300 ml of 20% strength sodium hydrogensulfite solutionand sat. sodium chloride solution. The organic phase is dried overmagnesium sulfate and concentrated under reduced pressure, and theresidue is purified on silica gel using n-heptane/ethyl acetate 3/1.This gives4-[5-(tert-butyldimethylsilanyloxy)-5-(4-fluorophenyl)-1-(4-methoxyphenyl)-2-(2-oxo-4-phenyloxazolidine-3-carbonyl)pentylamino]benzonitrileof molecular weight 707.93 (C₄₁H₄₆FN₃O₅Si); MS (ESI): 590.51(MH⁺-C₇H₅N₂);

e)4-[3-[3-(tert-Butyldimethylsilanyloxy)-3-(4-fluorophenyl)propyl]-2-(4-methoxyphenyl)-4-oxo-azetidin-1-yl]benzonitrile(40)

13.2 g. of4-[5-(tert-butyldimethylsilanyloxy)-5-(4-fluorophenyl)-1-(4-methoxyphenyl)-2-(2-oxo-4-phenyloxazolidine-3-carbonyl)pentylamino]benzonitrileare dissolved in 380 ml of methyl tert-butyl ether, 18.6 ml ofN,O-bis(trimethylsilyl)acetamide and 1.86 ml of a 1 M solution oftetrabutylammonium fluoride in tetrahydrofuran are added and the mixtureis stirred at room temperature for 2 h. After the reaction has ended, 10ml of acetic acid are added, the reaction mixture is concentrated underreduced pressure and the residue is purified on silica gel usingtoluene/ethyl acetate 50/1. This gives4-[3-[3-(tert-butyldimethylsilanyloxy)-3-(4-fluorophenyl)propyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzonitrileof molecular weight 544.75 (C₃₂H₃₇FN₂O₃Si); MS (ESI): 545.56 (M+H⁺).

f)4-[3-[3-(4-Fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzonitrile(41)

3.5 g of4-[3-[3-(tert-butyldimethylsilanyloxy)-3-(4-fluorophenyl)propyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzonitrileare dissolved in 65 ml of tetrahydrofuran, 0.74 ml of acetic acid and8.03 ml of a 1 M solution of tetrabutylammonium fluoride intetrahydrofuran are added, and the mixture is stirred at roomtemperature for 2 h. Another 4.82 ml of the tetrabutylammonium fluoridesolution are then added, and the mixture is stirred at refluxtemperature for another 3 h. The cooled reaction mixture is concentratedunder reduced pressure and the residue is purified chromatographicallyon silica gel using n-heptane/ethyl acetate 2/1. This gives4-[3-[3-(4-fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxo-azetidin-1-yl]benzonitrileof molecular weight 430.48 (C₂₆H₂₃FN₂O₃); MS (ESI): 431.24 (M+H⁺).

g)1-(4-Aminomethylphenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-methoxy-phenyl)azetidin-2-one(42)

1.22 g of4-[3-[3-(4-fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzonitrileare dissolved in 90 ml of ethanol, 10 ml of conc. ammonia solution andan excess of Raney nickel are added and the mixture is stirred at 60° C.and a hydrogen pressure of 10 bar for 8 h. Overnight, the reactionmixture cools to room temperature. The next day, the catalyst isseparated off, the filtrate is concentrated under reduced pressure andthe residue is purified chromatographically on silica gel usingdichloromethane/methanol/ammonia solution 10/1/0.1. This gives1-(4-aminomethylphenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-methoxyphenyl)azetidin-2-oneof molecular weight 434.51 (C₂₆H₂₇FN₂O₃); MS (ESI): 418.2 (MH⁺-NH₃).

h)Benzyl-(4-{4-[3-[3-(4-fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzylcarbamoyl}butyl)-(2,3,4,5,6-pentahydroxyhexyl)ammoniumtrifluoroacetate (35)

At room temperature, 100 mg of5-[Benzyl-(2,3,4,5,6-pentahydroxyhexyl)amino]-pentanoic acid and 1.10 mgof1-(4-aminomethylphenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-methoxyphenyl)azetidin-2-oneare dissolved in 2 ml of dry dimethylformamide, 42 mg ofN-hydroxybenzotriazole and 52 mg of1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride are addedand the mixture is stirred at room temperature overnight. The next day,the reaction mixture is concentrated under reduced pressure and, forpurification, chromatographed on RP18 using acetonitrile/water with 0.1%trifluoroacetic acid. This givesbenzyl-(4-{4-[3-[3-(4-fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzylcarbamoyl}butyl)-(2,3,4,5,6-pentahydroxyhexyl)ammoniumtrifluoroacetate of molecular weight 787.92 (C₄₄H₅₄FN₃O₉; cation); MS(ESI): 788.70 (M+H⁺).

EXAMPLE XIX

N-4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzyl-5-[benzyl-(2,3,4,5,6-pentahydroxyhexyl)amino]pentanamide(43)

The compound of Example XIX is prepared starting from5-[benzyl-(2,3,4,5,6-pentahydroxyhexyl)amino]pentanoic acid and4-(4-aminomethylphenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]azetidin-2-onesimilarly to the compound of Example XVIII. This givesN-4-{1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzyl-5-[benzyl-(2,3,4,5,6-pentahydroxyhexyl)amino]pentanamideof molecular weight 775.89 (C₄₃H₅₁F₂N₃O₈); MS (ESI): 776.4 (M+H⁺).

EXAMPLE XX

N-{4-[3-[3-(4-Fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzyl}acetamide(44)

The compound of Example XX is prepared by reacting acetic acid similarlyto Example XVIII with1-(4-aminomethylphenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-methoxyphenyl)azetidin-2-one.This givesN-{4-[3-[3-(4-fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzyl}acetamideof molecular weight 476.55 (C₂₈H₂₉FN₂O₄); MS (ESI): 477.22 (M+H⁺).

EXAMPLE XXI

5-(9H-Fluoren-9-ylmethoxycarbonylamino)-5-(4-{1-(4-fluorophenyl)-3-[3-(4-fluoro-phenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzylcarbamoyl)pentyl]trimethyl-ammoniumchloride (45)

The compound of Example XXI is obtained similarly to the procedure ofExample XIX by reacting4-(4-aminomethylphenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]azetidin-2-onewith[5-carboxy-5-(9H-fluoren-9-ylmethoxycarbonylamino)pentyl]trimethylammoniumchloride. This gives[5-(9H-fluoren-9-ylmethoxycarbonylamino)-5-(4-{1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzylcarbamoyl)pentyl]trimethylammoniumchloride of molecular weight 815.99 (C₄₉H₅₃F₂N₄O₅; cation); MS (ESI):815.81 (M+).

EXAMPLE XXII[5-Amino-5-(4-{1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzylcarbamoyl)pentyl]trimethylammoniumchloride hydrochloride (46)

110 mg of the compound of Example XXI are dissolved in 2 ml of drydimethylformamide, and 0.1 ml of piperidine are added. The reactionmixture is stirred at room temperature for 2 h and, after the reactionhas ended, concentrated under reduced pressure. The residue is stirredin water, filtered off with suction and washed with water, and thefiltrate is acidified with 2 N hydrochloric acid. The mixture isconcentrated under reduced pressure and the residue is dried under highvacuum. The crude product is suspended in dichloromethane, the organicphase is decanted off and the residue is taken up in methanol,concentrated under reduced pressure and dried under high vacuum. Thisgives [5-amino-5-(4-{1-(4-fluorophenyl)3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzylcarbamoyl)-pentyl]trimethylammoniumchloride hydrochloride of molecular weight 593.74 (C₃₄H₄₃F₂N₄O₃;cation); MS (ESI): 593.37 (M⁺).

3-[2-[(4-Bromophenyl)-(4-fluorophenylamino)methyl]-5-(tert-butyldimethylsilanyloxy-5-(4-fluorophenyl)pentanoyl]-4-phenyloxazolidin-2-one(47)

4.4 g of3-[5-(tert-butyldimethylsilanyloxy)-5-(4-fluorophenyl)pentanoyl]-4-phenyloxazolidin-2-oneare dissolved in 40 ml of absolute dichloromethane. 5.2 g of(4-bromobenzylidene)-(4-fluorophenyl)amine and 8.6 ml ofethyldiisopropylamine are added, and the solution is then cooled to −10°C. 2.94 ml of trimethylsilyl chloride are then added dropwise, andduring the addition, the temperature of the reaction mixture ismaintained below −5° C. The reaction solution is then stirred at −10° C.for half an hour and then cooled to −30° C., and 1.2 ml of titaniumtetrachloride are added dropwise, the temperature being maintainedbetween −30° C. and −15° C. This gives a black reaction solution whichis stirred at −20° C. for another 3 h and then allowed to warm to 0° C.In the stated order, in intervals of 10 minutes, 10 ml of glacial aceticacid, 100 ml of 7% strength aqueous tartaric acid solution and finally100 ml of 20% strength aqueous sodium hydrogensulfite solution are addedwith stirring. The mixture is then extracted twice with dichloromethaneand the organic phase is washed once with saturated sodium chloridesolution and dried over sodium sulfate. The solvent is removed using arotary evaporator and the residue is purified by column chromatography(SiO₂; ethyl acetate/heptane 1:4). The product is obtained from diethylether/pentane as white crystals. C₃₉H₄₃BrF₂N₂O₄Si (749) MS (ESI): M⁺

4-(4-Bromophenyl)-3-[3-(tert-butyldimethylsilanyloxy)-3-(4-fluorophenyl)propyl]-1-(4-fluorophenyl)azetidin-2-one(48)

3.34 g of3-[2-[(4-bromophenyl)-(4-fluorophenylamino)methyl]-5-(tert-butyl-dimethylsilanyloxy)-5-(4-fluorophenyl)pentanoyl]-4-phenyloxazolidin-2-oneare suspended in 70 ml of tert-butyl methyl ether. 3.8 ml ofbis(trimethylsilyl)acetamide and 144 mg of tributylammonium fluoridetrihydrate are then added. The reaction mixture is stirred at roomtemperature overnight, and 0.7 ml of glacial acetic acid are then added.The reaction mixture is concentrated using a rotary evaporator and theresidue is purified by column chromatography (SiO₂; ethylacetate/heptane 1:4). The product is, obtained as a clear oil.C₃₀H₃₄BrF₂NO₂Si (586) MS (ESI): M⁺-131

3-{5-(tert-Butyldimethylsilanyloxy)-5-(4-fluorophenyl)-2-[(4-fluorophenylamino)-(4-hydroxyphenyl)methyl]pentanoyl}-4-phenyloxazolidin-2-one(49)

10 g of3-[5-(tert-butyldimethylsilanyloxy)-5-(4-fluorophenyl)pentanoyl]-4-phenyloxazolidin-2-oneare dissolved in 80 ml of absolute dichlormethane. 9.12 g of4-[(4-fluorophenylimino)methyl]phenol and 19.6 ml ofethyldiisopropylamine are added, and the solution is then cooled to −10°C. 6.7 ml of trimethylsilyl chloride are then added dropwise, thetemperature of the reaction mixture being maintained at below −5° C. Thereaction solution is stirred at −10° C. for half an hour and then cooledto −30° C., and 2.7 ml of titanium tetrachloride are added dropwise, thetemperature being maintained between −30° C. and −15° C. This gives ablack reaction solution which is stirred at −20° C. for another 3 h andthen allowed to warm to 0° C. In the stated order, in intervals of 10minutes, 6 ml of glacial acetic acid, 60 ml of 7% strength aqueoustartaric acid solution and finally 100 ml of 20% strength aqueous sodiumhydrogensulfite solution are then added with stirring. The mixture isthen extracted three times with dichloromethane and the organic phase iswashed once with saturated sodium chloride solution and dried oversodium sulfate. The solvent is removed using a rotary evaporator and theresidue is purified by column chromatography (SiO₂; ethylacetate/heptane 1:4). The product is obtained from diethyl ether/pentaneas white crystals. C₃₉H₄₄F₂N₂O₅Si (686) MS (ESI): M⁺-241

3-[3-(tert-Butyldimethylsilanyloxy)-3-(4-fluorophenyl)propyl]-1-(4-fluorophenyl)-4-(4hydroxyphenyl)azetidin-2-one (50)

2.63 g of3-{5-(tert-butyldimethylsilanyloxy)-5-(4-fluorophenyl)-2-[(4-fluorophenylamino)-(4-hydroxyphenyl)methyl]pentanoyl}-4-phenyloxazolidin-2-one aresuspended in 60 ml of tert-butyl methyl ether. 3.22 ml ofbis(trimethylsilyl)acetamide and 122 mg of tributylammonium fluoridetrihydrate are then added. The reaction mixture is stirred at roomtemperature for 3 h, and 0.6 ml of glacial acetic acid are then added.The reaction mixture is concentrated using a rotary evaporator and theresidue is purified by column chromatography (SiO₂; ethylacetate/heptane 1:4). The product is obtained as clear crystals.C₃₀H₃₅F₂NO₃Si (523) MS (ESI): M⁺-131

[3-(4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}phenoxy)propyl]trimethylammoniumbromide (51)

210 mg of3-[3-(tert-butyldimethylsilanyloxy)-3-(4-fluorophenyl)propyl]-1-(4-fluorophenyl)-4-(4-hydroxyphenyl)azetidin-2-oneare dissolved in 4 ml of absolute acetonitrile. 170 mg of KF-alumina(1.15 mol/100) and 200 mg of (3-bromopropyl) trimethylammonium bromideare then added. The reaction mixture is stirred at room temperature for4 h and then filtered. The mother liquor is concentrated using a rotaryevaporator and the residue is purified using a 5 g SiO₂ cartridge(dichloromethane/methanol 5:1). The product is obtained as an oil.

C₃₆H₄₉BrF₂N₂O₃Si (703) MS (ESI): M⁺-80

EXAMPLE XXIII[3-(4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}phenoxy)propyl]trimethylammoniumbromide (52)

180 mg of(3-{4-[3-[3-(tert-butyldimethylsilanyloxy)-3-(4-fluorophenyl)propyl]-1-(4-fluorophenyl)-4-oxoazetidin-2-yl]phenoxy}propyl)trimethylammoniumbromide are dissolved in 10 ml of methanol. 1 ml of a 0.1 M aqueous HClsolution is then added, and the reaction solution is stirred at roomtemperature overnight. The mixture is neutralized with dilute aqueoussodium bicarbonate solution and concentrated using a rotary evaporator.The residue is purified using a 10 g SiO₂ cartridge(dichloromethane/methanol 5:1). The product is obtained as a hygroscopicsolid. C₃₀H₃₅BrF₂N₂O₃ (589) MS (ESI): M⁺-80

[5-(4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-(tert-butyldimethylsilanyloxy)propyl]-4-oxoazetidin-2-yl}phenoxy)pentyl]trimethylammoniumbromide (53)

370 mg of3-[3-(tert-butyldimethylsilanyloxy)-3-(4-fluorophenyl)propyl]-1-(4-fluorophenyl)-4-(4-hydroxyphenyl)azetidin-2-oneare dissolved in 3 ml of absolute acetonitrile. 300 mg of KF-alumina(1.15 mol/100 g) and 375 mg of (3-bromopentyl)-trimethylammonium bromideare then added. The reaction mixture is stirred at room temperatureovernight and then filtered. The mother liquor is concentrated using arotary evaporator and the residue is purified using a 5 g SiO₂ cartridge(dichloromethane/methanol 4:1). The product is obtained as an oil.

C₃₈H₅₃BrF₂NO₃Si (731) MS (ESI): M⁺-80

EXAMPLE XXIV[5-(4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}phenoxy)pentyl]trimethylammoniumbromide (54)

548 mg of[5-(4-{1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-(isopropyldimethyl-silanyloxy)propyl]-4-oxoazetidin-2-yl}phenoxy)pentyl]trimethylammoniumbromide are dissolved in 20 ml of methanol. 1 ml of a 0.1 M aqueous HClsolution is then added, and the reaction solution is stirred at roomtemperature overnight. The mixture is neutralized with dilute aqueoussodium bicarbonate solution and concentrated using a rotary evaporator.The residue is purified using a 10 g SiO₂ cartridge(dichloromethane/methanol 5:1). The product is obtained as a hygroscopicsolid. C₃₂H₃₉BrF₂N₂O₃ (617) MS (ESI): M⁺-80

1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-[4-(4-iodobutoxy)phenyl]azetidin-2-one(55)

100 mg of1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-hydroxy-phenyl)azetidin-2-oneare dissolved in 10 ml of absolute dimethylformamide. 80 mg of powderedpotassium carbonate and 0.2 ml of diiodobutane are then added. Thereaction solution is stirred at room temperature overnight. Followingconcentration using a rotary evaporator and oil pump vacuum at 40° C.,the residue is purified using an SiO₂ cartridge (n-heptane;n-heptane/ethyl acetate 4:1). The product is obtained as an oil.C₂₈H₂₈F₂INO₃ (591) MS (ESI): M⁺-18

EXAMPLE XXV1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-{4-[methyl-(2,3,4,5,6-pentahydroxyhexyl)amino]butoxy}phenyl)azetidin-2-one(56)

100 mg of1′-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-[4-(4-iodobutoxy)phenyl]azetidin-2-oneare dissolved in 5 ml of absolute dimethylformamide. 132 mg of6-methylaminohexane-1,2,3,4,5-pentaol are then added, and the reactionsolution is stirred at 50° C. for 2 h. After concentration using arotary evaporator and oil pump vacuum at 40° C., the residue is purifiedby preparative HPLC. The product (89 mg) is obtained as an oil.C₃₅H₄₄F₂N₂O₈ (658) MS (ESI): M⁺

1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-[4-(5-iodopentyloxy)-phenyl]azetidin-2-one(57)

150 mg of1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-hydroxyphenyl)azetidin-2-oneare dissolved in 10 ml of absolute dimethylformamide. 120 mg of powderedpotassium carbonate and 0.33 ml of diiodopentane are then added. Thereaction solution is stirred at room temperature overnight. Afterconcentration using a rotary evaporator and oil pump vacuum at 40° C.,the residue is purified using an SiO₂ cartridge (n-heptane;n-heptane/ethyl acetate 4:1). The product is obtained as an oil.C₂₉H₃₀F₂INO₃ (605) MS (ESI): M⁺-18

EXAMPLE XXVI1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-{5-[methyl-(2,3,4,5,6-pentahydroxyhexyl)amino]pentyloxy}phenyl)azetidin-2-one(58)

170 mg of1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-[4-(5-iodopentyloxy)phenyl]azetidin-2-oneare dissolved in 5 ml of absolute dimethylformamide. 220 mg of6-methylaminohexane-1,2,3,4,5-pentaol are then added, and the reactionsolution is stirred at 50° C. for 2 h. After concentration using arotary evaporator and oil pump vacuum at 40° C., the residue is purifiedby preparative HPLC. The product is obtained as an oil. C₃₆H₄₆F₂N₂O₈(672) MS (ESI): M⁺

1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-[4-(6-iodohexyloxy)-phenyl]azetidin-2-one(59)

100 mg of 1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4hydroxyphenyl)azetidin-2-one are dissolved in 10 ml of absolutedimethylformamide. 80 mg of powdered potassium carbonate and 0.25 ml ofdiiodohexane are then added. The reaction solution is stirred at roomtemperature overnight. After concentration using a rotary evaporator andoil pump vacuum at 40° C., the residue is purified using an SiO₂cartridge (n-heptene; n-heptane/ethyl acetate 4:1). The product isobtained as an oil. C₃₀H₃₂F₂INO₃ (619) MS (ESI): M⁺-18

EXAMPLE XXVII1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-{6-[methyl-(2,3,4,5,6-pentahydroxyhexyl)amino]hexyloxy}phenyl)azetidin-2-one(60)

136 mg of1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-[4-(6-iodohexyloxy)phenyl]azetidin-2-oneare dissolved in 10 ml of absolute dimethylformamide. 172 mg of6-methylaminohexane-1,2,3,4,5-pentaol are then added, and the reactionsolution is stirred at 50° C. for 2.5 h. After concentration using arotary evaporator and oil pump vacuum at 40° C., the residue is purifiedby preparative HPLC. The product is obtained as an oil. C₃₇H₄₈F₂N₂O₈(686) MS (ESI): M⁺

1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-[4-(8-iodooctyloxy)-phenyl]azetidin-2-one(61)

150 mg of1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-hydroxyphenyl)azetidin-2-oneare dissolved in 10 ml of absolute dimethylformamide. 120 mg of powderedpotassium carbonate and 0.44 ml of diiodooctane are then added. Thereaction solution is stirred at room temperature overnight. Afterconcentration using a rotary evaporator and oil pump vacuum at 40° C.,the residue is purified using an SiO₂ cartridge (n-heptane;n-heptane/ethylacetate 4:1). The product is obtained as an oil.C₃₂H₃₆F₂INO₃ (647) MS (ESI): M⁺-18

EXAMPLE XXVIII1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-{8-[methyl-(2,3,4,5,6-pentahydroxyhexyl)amino]octyloxy}phenyl)azetidin-2-one(62)

150 mg of1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-[4-(8-iodooctyloxy)phenyl]azetidin-2-oneare dissolved in 5 ml of absolute dimethylformamide. 180 mg of6-methylaminohexane-1,2,3,4,5-pentaol are then added, and the reactionsolution is stirred at 50° C. for 2 h. After concentration using arotary evaporator and oil pump vacuum at 40° C., the residue is purifiedby preparative HPLC. The product is obtained as an oil. C₃₉H₅₂F₂N₂O₈(714) MS (ESI): M⁺

1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-[4-(10-iododecyloxy)-phenyl]azetidin-2-one(63)

150 mg of 1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4hydroxyphenyl)azetidin-2-one are dissolved in 10 ml of absolutedimethylformamide. 120 mg of powdered potassium carbonate and 865 mg ofdiiododecane are then added. The reaction solution is stirred at roomtemperature overnight. After concentration using a rotary evaporator andoil pump vacuum at 40° C., the residue is purified using an SiO₂cartridge (n-heptane; n-heptane/ethyl acetate-4:1). The product isobtained as an oil. C₃₄H₄₀F₂INO₃ (675) MS (ESI): M⁺-18

EXAMPLE XXIX1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-{10-[methyl-(2,3,4,5,6-pentahydroxyhexyl)amino]decyloxy}phenyl)azetidin-2-one(64)

170 mg of1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-[4-(10-iododecyloxy)phenyl]azetidin-2-oneare dissolved in 5 ml of absolute dimethylformamide. 200 mg of6-methylaminohexane-1,2,3,4,5-pentaol are then added, and the reactionsolution is stirred at 50° C. for 2 h. After concentration using arotary evaporator and oil pump pressure at 40° C., the residue ispurified by preparative HPLC. The product is obtained as an oil.C₄₁H₅₆F₂N₂O₈ (742) MS (ESI): M⁺

1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-{2-[2-(2-iodoethoxy)ethoxy]ethoxy}phenyl)azetidin-2-one(5)

150 mg of 1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4hydroxyphenyl)azetidin-2-one are dissolved in 10 ml of absolutedimethylformamide. 120 mg of powdered potassium carbonate and 0.4 ml of1,2-bis(diiodoethoxy)ethane are then added. The reaction solution isstirred at room temperature overnight. After concentration using arotary evaporator and oil pump vacuum at 40° C., the residue is purifiedusing an SiO₂ cartridge (n-heptane; n-heptane/ethylacetate 4:1). Theproduct is obtained as an oil. C₃₀H₃₂F₂INO₅ (651) MS (ESI): M⁺-18

EXAMPLE XXX1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-{4-[2-(2-{2-[methyl-(2,3,4,5,6-pentahydroxyhexyl)amino]ethoxy}ethoxy)ethoxy]phenyl}azetidin-2-one(66)

230 mg of1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-{2-[2-(2-iodoethoxy)ethoxy]ethoxy}phenyl)azetidin-2-oneare dissolved in 5 ml of absolute dimethylformamide. 280 mg of6-methylaminohexane-1,2,3,4,5-pentaol are then added, and the reactionsolution is stirred at 50° C. for 2 h. After concentration using arotary evaporator and oil pump vacuum at 40° C., the residue is purifiedby preparative HPLC. The product is obtained as an oil. C₃₇H₄₈F₂N₂O₁₀(718) MS (ESI): M⁺

N-methyl-N-(2,3,4,5,6-pentahydroxyhexyl)hex-5-enamide (67)

1.11 g of 5-hexenoic acid are dissolved in 3 ml of absolute methylenedichloride. 1.4 ml of thionyl chloride are then added dropwise. Themixture is stirred at room temperature for 3 h and then concentratedusing a rotary evaporator. 1.09 g of6-methylaminohexane-1,2,3,4,5-pentaol are suspended in 5 ml of absolutemethylene dichloride. 5-hexenoic chloride dissolved in 3 ml of absolutemethylene dichloride is added dropwise, and the mixture is then stirredat room temperature for 4 h. The resulting precipitate is filtered offfrom the reaction product, the filtrate is concentrated using a rotaryevaporator and the oily crude product is reacted further without anypurification. C₁₃H₂₅NO₆ (291) MS (ESI): M⁺

N-methyl-N-(2,3,4,5,6-pentahydroxyhexyl)-6-{4-[3-[3-(tert-butyldimethylsilanyloxy)-3-(4-fluorophenyl)propyl]-1-(4-fluorophenyl)-4-oxoazetidin-2-yl]phenyl}hex-5-enamide(68)

110 mg of4-(4-bromophenyl)-3-[3-(tert-butyldimethylsilanyloxy)-3-(4-fluorophenyl)propyl]-1-(4-fluorophenyl)azetidin-2-oneand 136 mg of N-methyl-N-(2,3,4,5,6-pentahydroxyhexyl)hex-5-enamide areinitially charged in 300 μl of triethylamine under argon in a closedtube which had been heated thoroughly beforehand. After addition of 6 mgof palladium acetate and 14 mg of triphenylphosphine, the mixture isstirred at 100° C. for 4 h. The reaction mixture is then taken up indichloromethane, filtered and concentrated using a rotary evaporator.Purification of the residue using an SiO₂ cartridge(dichloromethane/methanol 20:1-5:1) gives the product. C₄₃H₅₈F₂N₂O₈Si(796)

EXAMPLE XXXIN-methyl-N-(2,3,4,5,6-pentahydroxyhexyl)-6-(4-{1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}phenyl)hex-5-enamide(69)

70 mg ofN-methyl-N-(2,3,4,5,6-pentahydroxyhexyl)-6-{4-[3-[3-(tert-butyldimethylsilanyloxy)-3-(4-fluorophenyl)propyl]-1-(4-fluorophenyl)-4-oxoazetidin-2-yl]phenyl}hex-5-enamideare dissolved in 6 ml of methanol. 0.1 N HCl_((aq)) is then added, andthe mixture is stirred at room temperature overnight. The mixture isthen neutralized with 1 N aqueous sodium hydroxide solution andconcentrated using a rotary evaporator. The residue is stirred withdichloromethane and filtered and the mother liquor is concentrated usinga rotary evaporator. The product is obtained following purification bypreparative HPLC: C₃₁H₄₄F₂N₂O₈ (682) MS (ESI): M⁺-18

EXAMPLE XXXII2-{[4-(4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}phenoxy)butyl]methylamino}ethanesulfonicacid (70)

64.5 mg of1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-[4-(4iodobutoxy)phenyl]azetidin-2-one are dissolved in 3 ml of methanol. 60.7mg of 2-methylaminoethanesulfonic acid are then dissolved in 1 ml ofwater, and 30.4 mg of potassium carbonate are added. The reactionsolution is stirred at 50° C. for 8 h. After concentration using arotary evaporator at 40° C., the residue is passed over a reverse-phasecartridge (methanol). The resulting crude product is dissolved in hotmethanol. The precipitate formed on cooling is filtered off, and themother liquor is concentrated using a rotary evaporator. The product isobtained as an oil.

C₃₁H₃₆F₂N₂O₆S (602) MS (ESI): M⁺-18

EXAMPLE XXXIII1-(4-Fluorophenyl)-3-[1-(4-fluorophenyl)-2-oxo-4-(4-sulfoxyphenyl)azetidin-3-yl]propylacetate (71)

120 mg (0.27 mmol) of1-(4-fluorophenyl)-3-[1-(4-fluorophenyl)-2-(4-hydroxyphenyl)-4-oxoazetidin-3-yl]propylacetate are dissolved in 3 ml of pyridine, and 200 mg of Me₃NSO₃ complex(Aldrich) are added. The suspension is stirred at room temperature for30 hours. The mixture is then diluted with 5 ml of methylenechloride/methanol/conc. ammonia (30/5/1) and purified by flashchromatography using the same solvent mixture. The product is obtainedas an amorphous solid.

C₂₆H₂₃F₂NO₇S (531.54) MS (ESI): M⁺=532.2.

EXAMPLE XXXIVMono-(4-{1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}phenyl)sulfate(72)

75 mg (0.14 mmol) of1-(4-fluorophenyl)-3-[1-(4-fluorophenyl)-2-oxo-4-(4-sulfoxy-phenyl)azetidin-3-yl]propylacetate are dissolved in 2 ml of methanol, and 0.3 ml of 1 N NaOMe/MeOHis added. After 2 hours at room temperature, the mixture is neutralizedwith methanolic hydrochloric acid and concentrated. The residue ispurified by flash chromatography. The product is obtained as anamorphous solid.

C₂₄H₂₁F₂NO₆S (489.50) MS (ESI): M⁺=490.2.

EXAMPLE XXXV2,3,4,5-Tetraacetoxy-1-{3-[3-[3-(4-fluorophenyl)-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzylcarbamoyl}pentylacetate (73)

112 mg (0.24 mmol) of1-(3-aminomethylphenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]4-(4-methoxyphenyl)azetidin-2-oneare dissolved in 5 ml of methylene chloride and 0.5 ml of triethylamine.At 0° C., 0.5 g of 2,3,4-triacetoxy-1-(acetoxychlorocarbonylmethyl)butylacetate are added, and the mixture is allowed to thaw to roomtemperature. After 30 minutes, the mixture is diluted with ethyl acetateand then filtered through silica gel. The solvent is distilled off andthe residue is purified by flash chromatography. The product is obtainedas an amorphous solid:

C₄₂H₄₇FN₂O₁₄ (822.84) MS (ESI): M⁺=823.3.

EXAMPLE XXXVIN-3-[3-[3-(4-Fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzyl-2,3,4,5,6-pentahydroxyhexanamide(74)

90 mg-(109 μmol) of2,3,4,5-tetraacetoxy-1-{3-[3-[3-(4-fluorophenyl)-3-hydroxy-propyl]-2-(4-methoxyphenyl-4-oxoazetidin-1-yl]benzylcarbamoyl}pentylacetate are dissolved in 7 ml of methanol and 0.5 ml of 1 N NaOMe/MeOHis added. After 2 hours at room temperature the mixture is neutralizedwith methanolic hydrochloric acid and concentrated. The residue ispurified by flash chromatography. The product is obtained as anamorphous solid. C₃₂H₃₇FN₂O₉ (612.66) MS (ESI): M⁺=613.2.

EXAMPLE XXXVIIN-Methyl-N-(2,3,4,5,6-pentahydroxyhexyl)-6-(4-{3-[1-(4-fluorophenyl)-2-(4-methoxy-phenyl)-4-oxoazetidin-3-yl]-1-hydroxypropyl}phenyl)hex-5-enamide(75)

200 mg of N-methyl-N-(2,3,4,5,6-pentahydroxyhexyl)hex-5-enamide and 72mg of3-[3-(4-bromophenyl)-3-hydroxypropyl]-1-(4-fluorophenyl)-4-(4-methoxyphenyl)-azetidin-2-oneare prepared analogously to the synthesis of Example XXXI. The productis obtained as an amorphous solid.

Using the method described below, the activity of the compounds of theformula I according to the invention was examined:

Effect on Cholesterol Absorption 4 ³H-Taurocholic Acid Excretion UsingFecal Excrement of Mice, Rats or Hamsters

NMRI mice, Wistar rats, or Golden Syrian hamsters (in groups of n=4−6)are kept in metabolic cages, where they are fed with a standard diet(Altromin, Lage (Lippe)). The afternoon prior to the administration ofthe radioactive tracers (¹⁴C-cholesterol), the feed is removed and theanimals are adapted to grates.

Additionally, the animals are labeled s.c. with ³H-TCA (taurocholicacid) (for example 1 μCi/mouse up to 5 μCi/rat) 24 hours prior to theperoral administration of the test meal (¹⁴C-cholesterol in Intralipid®20, Pharmacia-Upjohn).

Cholesterol absorption test: 0.25 ml/mouse Intralipid® 20(Pharmacia-Upjohn) ((spiked with 0.25 μCi of ¹⁴C-cholesterol in 0.1 mgof cholesterol) is administered perorally by gavage.

Test substances are prepared separately in 0.5% methylcellulose(Sigma)/5% Solutol (BASF, Ludwigshafen) or a suitable vehicle.

The administration volume of the test substance is 0.5 ml/mouse. Thetest substance is administered immediately prior to the test meal(Intralipid labeled with ¹⁴C-cholesterol) (cholesterol absorption test).

The feces are collected over a period of 24 h: fecal elimination of¹⁴C-cholesterol and ³H-taurocholic acid (TCA) is determined after 24hours.

The livers are removed and homogenized, and aliquots are incinerated inan oximate (Model 307, Packard) to determine the amount of¹⁴C-cholesterol which had been taken up/absorbed.

Evaluation: Feces Samples:

The total weight is determined, the sample is made up with water to adefined volume and then homogenized, and an aliquot is evaporated todryness and incinerated in an oximate (Model 307 from Packard for theincineration of radioactively labeled samples): the amount ofradioactive ³H—H₂O and ¹⁴C—CO₂ is extrapolated to the amount of³H-taurocholic acid and ¹⁴C-cholesterol, respectively, that is excreted(dual isotope technique). The ED₂₀₀ values as dose from a dose-effectcurve are interpolated as those doses at which the excretion of TCA orcholesterol is doubled, based on a control group treated at the sametime.

Liver Samples:

The amount of ¹⁴C-cholesterol taken up by the liver is based on theadministered dose. The EDN values are interpolated from a dose-effectcurve as the dose at which the uptake of ¹⁴C-cholesterol by the liver ishalved (50%), based on a control group.

The ED₅₀ values below demonstrate the activity of the compounds of theformula I according to the invention

Example No. ED₅₀ (liver) [mg/mouse] II 0.1 III 0.003 XIII 0.3 XV 0.01XVIII 1.0 XX 0.03 XXI 1.0 XXIV 0.3 XXV 0.3 XXX 0.1

As can be seen from the table, the compounds of the formula I have verygood cholesterol-lowering action. The compounds can thus be used tocontrol cholesterol concentration. Such control can be by lowering thecholesterol concentration, or maintaining a desired level of cholesterolconcentration.

Bioabsorption:

The bioabsorption of the compounds of the formula I can be examinedusing the Caco cell model (A. R. Hilgers et al., Caco-2 cell monolayersas a model for drug transport across the intestinal mucosa, Pharm. Res.1990, 7, 902).

Below is a bioabsorption measurement for a reference compound:

Reference structure Example Apparent partition coefficient 4.88 × 10⁻⁰⁶P_(app) [cm/s] (according to Lit. Hilgers) Estimated human bioabsorption100%

Reference structure:Ezetimibe

1-17. (canceled) 18.1-(4-aminomethylphenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-methoxyphenyl)azetidin-2-one.